TABLE 3.
Detection of anthrax PA in mice injected with PA, LF, and EFa
| Toxin(s) | Bleeding time (h) | No. of animals | PA concn in blood (μg/ml) measured by:
|
|
|---|---|---|---|---|
| ENIA | ELISA | |||
| PA | 2 | 3 | 1.60 ± 0.40b | 2.23 ± 0.31 |
| PA | 6 | 2 | 1.14 ± 1.51 | 0.94 ± 1.08 |
| PA + LF | 2 | 3 | 1.23 ± 0.15 | 1.33 ± 0.08 |
| PA + LF | 6 | 3 | 0.70 ± 0.10 | 1.10 ± 0.15 |
| LF | 2 | 3 | 0.00 | 0.00 |
| LF | 6 | 3 | 0.00 | 0.00 |
| EF | 2 | 3 | 0.00 | 0.00 |
| EF | 6 | 3 | 0.00 | 0.00 |
a
BALB/cJ mice were injected intravenously (200 μl/vein) with 50 μg of wild-type PA, LF, or EF or with premixed combinations of these toxins (all prepared in PBS) and were bled 2 or 6 h after injection. The PA levels in plasma were assessed by the ENIA and ELISA methods. The samples were diluted from 1:10 to 1:1,000.
b
The data are means ± SDs.