TABLE 2.

Sensitivity of qRT-PCR and microarray assay for each virus

Virus	Gene(s)a	qRT-PCR assay		Microarray assay
		Thresholdb	Sensitivity (copy no.)	Thresholdc	Sensitivity (copy no.)
Adenovirus	IVA2/L4100kd	40	10	1.5	1,000 (group B)/250 (group C)
Coronavirus 229E	Nucleocapsid	45	50	3	2,500
Coronavirus HKU1	Nucleocapsid	40	10	1.5	500
Coronavirus NL63	Nucleocapsid	40	100	1.5	500
Coronavirus OC43	Nucleocapsid	45	50	2	250
Enterovirus	5′ untranslated region	40	5	1/1.5	250
Rhinovirus	5′ untranslated region	40	250	1/1.5	1,000
Influenza virus A	Nucleocapsid	40	50	1.5	100
Influenza virus B	Nucleocapsid	42	10	1.5	100
HMPV A	Matrix/F	40	50	1	10
HMPV B	Matrix/F	40	50	3	250
HRSV A	Nucleocapsid	45	250	1	50
HRSV B	Nucleocapsid	45	10	1	10
Parainfluenza virus 1	Hemagglutinin-neuraminidase	43	50	2	5,000
Parainfluenza virus 2	Fusion	40	5	2	2,500
Parainfluenza virus 3	Hemagglutinin-neuraminidase	44	50	2	2,500

^aA slash shows target genes that differ in qRT-PCR/microarray assays.

^bMaximum cycle threshold for positivity.

^cMinimum ratio for positivity. A slash shows threshold for Picornaviridae detection/threshold for species-specific detection.