TABLE 5.
Comparison of qRT-PCR and microarray results for 221 specimens
| Virus | No. of specimens with indicated qRT-PCR/microarray resultsb
|
Microarrayc
|
||||
|---|---|---|---|---|---|---|
| Pos/pos | Pos/neg | Neg/pos | Neg/neg | Sensitivity | Specificity | |
| Adenovirusa | 9 | 7 | 0 | 205 | 0.563 | 1.000 |
| Coronavirus 229E | 0 | 0 | 0 | 221 | NA | 1.000 |
| Coronavirus HKU1 | 8 | 1 | 0 | 212 | 0.889 | 1.000 |
| Coronavirus NL63 | 5 | 2 | 0 | 214 | 0.714 | 1.000 |
| Coronavirus OC43 | 3 | 0 | 0 | 218 | 1.000 | 1.000 |
| Enterovirus/rhinovirus | 28 | 1 | 0 | 192 | 0.966 | 1.000 |
| Influenza virus A | 28 | 1 | 0 | 192 | 0.966 | 1.000 |
| Influenza virus B | 0 | 0 | 0 | 221 | NA | 1.000 |
| HMPV A | 4 | 1 | 0 | 216 | 0.800 | 1.000 |
| HMPV B | 4 | 0 | 0 | 217 | 1.000 | 1.000 |
| HRSV A | 22 | 0 | 0 | 199 | 1.000 | 1.000 |
| HRSV B | 84 | 0 | 0 | 137 | 1.000 | 1.000 |
| Parainfluenza virus 1 | 1 | 0 | 0 | 220 | 1.000 | 1.000 |
| Parainfluenza virus 2 | 1 | 0 | 0 | 220 | 1.000 | 1.000 |
| Parainfluenza virus 3 | 1 | 0 | 0 | 220 | 1.000 | 1.000 |
| Any virus | 176 | 4 | 0 | 41 | 0.978 | 1.000 |
a
Results of qRT-PCR and microarray assays were significantly different only for adenovirus. (P < 0.05; McNemar's test).
b
Pos, positive; neg, negative.
c
NA, not applicable.