TABLE 6.

Signal, internal control, and DNA sequence validation results for specimens with discordant results in qRT-PCR and microarray assays

Specimen	qRT-PCR assay			Microarray assay			Validation sequencing result
	Viruses detecteda	Signal (CT)b of:		Viruses detected	Signal (ratio)c of:
		Discordant virus	Internal control		Discordant virus	Internal control
032	Coronavirus HKU1, HRSV B	38.04	35.03	HRSV B	0.92	3.51	Coronavirus HKU1
034	Influenza virus A	39.88	32.09	Negative	0.71	10.82	Influenza virus A
049	Adenovirus, influenza virus A, HRSV A	35.54	32.28	Influenza virus A, HRSV A	0	8.08	Adenovirus C
069	Coronavirus NL63, influenza virus A	39.27	30.8	Influenza virus A	2.44d	9	Coronavirus 229E
075	Adenovirus, HRSV B	37.97	31.16	HRSV B	0	11.33	Negative
081	Adenovirus	32.49	33.34	Negative	0	2.56	Negative
092	Adenovirus, influenza virus A	35.35	32.19	Influenza virus A	0	2.49	Negative
121	HMPV A	38.44	34.54	Negative	0	2.88	HMPV A
156	Coronavirus NL63, HRSV B	35.7	34.56	HRSV B	0	11.56	Coronavirus NL63
173	Adenovirus, rhinovirus	34.66	33.04	Rhinovirus A	0	8.53	Adenovirus C
182	Adenovirus, HRSV B	38.9	32.48	HRSV B	0.31	10.34	Negative
186	Adenovirus, rhinovirus	35.31	32.44	Rhinovirus A	0.33	8.86	Negative
218	Rhinovirus/enterovirus	38.9	37.68	Negative	0.78	9.71	Rhinovirus A

^aViruses with discordant results are shown in boldface.

^bC_T, cycle threshold.

^cRatio of results for virus or internal control/background.

^dAn equivocal signal was seen for coronavirus 229E.