TABLE 3.
Seven LVFX low-susceptibility/resistant strains detected by PCR-MCA
| Strain | MIC (μg/ml) to LVFX | Susceptibility at QRDRsa
|
||||||
|---|---|---|---|---|---|---|---|---|
|
parC codon 84
|
parC codon 88
|
gyrA codon 84
|
gyrA codon 88
|
|||||
| Tm (°C) | Sequence/aa | Sequence/aa | Tm (°C) | Sequence/aa | Tm (°C) | Sequence/aa | ||
| Rd (wild type) | 0.003 | 56.86 | AGT/Ser | GAA/Glu | 62.44 | TCC/Ser | 56.49 | GAT/Asp |
| DS-1 | 8 | 55.05 | AGC/Thr | WT | 52.04 | TTA/Leu | 53.81 | GGT/Gly |
| NUH-1 | 16 | 50.73 | AGA/Arg | GCG/Ala | 52.04 | TTA/Leu | 53.81 | AAT/Asn |
| BY-1 | 8 | 50.73 | AGA/Arg | WT | 50.86 | TTG/Leu | 48.59 | AAT/Asn |
| MS24060 | 1 | 50.73 | AGA/Arg | GCG/Ala | 57.93 | GCC/Ala | 56.49 | WT |
| MS27995 | 1 | 52.11 | ATC/Ile | WT | 52.04 | TTA/Leu | 56.49 | WT |
| MS11438 | 4 | 50.73 | AGA/Arg | GCG/Ala | 52.04 | TTA/Leu | 48.59 | TAT/Tyr |
| DS-2 | 4 | 50.82 | AGA/Arg | GAT/Asp | 53.24 | TAC/Thr | 49.70 | TAT/Tyr |
a
aa, amino acid. WT, wild type.