TABLE 3.

Comparison of the endpoint detection limits of the multiplex and singleplex PCR assays

Virus and dilution	Detection limits of assaya
	Singleplex	Multiplex
CMV
10−1	23.94, 23.83	23.40, 23.73
10−2	27.35, 26.96	26.79, 27.24
10−3	32.20, 32.07	30.21, 30.34
10−4	33.39, 33.28	33.20, 34.09
10−5	36.27, −	37.43, −
EBV
10−1	23.19, 23.22	23.36, 23.34
10−2	26.37, 26.41	26.22, 26.25
10−3	30.02, 29.58	30.00, 29.52
10−4	32.92, 34.13	33.62, 32.89
10−5	37.13, −	35.47, −
Adenovirus
10−1	22.20, 22.63	22.40, 22.33
10−2	26.21, 26.60	25.51, 25.60
10−3	30.08, 29.64	29.08, 29.24
10−4	32.99, 33.59	32.58, 32.59
10−5	36.52, −	35.52, 35.09

^aExpressed as C_T values obtained with samples tested in duplicate. −, no virus detected.