TABLE 1.

Primers and probes

Primer or probea	Sequenceb (5′-3′)	Nucleotide position
E7 forward primer	CGGACAGAGCCCATTACAATATT	701-723
E7 reverse primer	CGCACAACCGAAGCGTAGA	766-748
E7-specific probe	FAM-TAACCTTTTGTTGCAAGTGT-MGBNFQ	725-744
E7 degenerate probe	FAM-TAACCTTYTGTTGCAAGTGT-MGBNFQ	725-744
E2-specific forward primer	GCCGCGACCCATACCAAA	3413-3430
E2 degenerate forward primer	GCCGCGACCCATWCCAAA	3413-3430
Modified E2 degenerate forward primer 1	CGCCGCGACCCATWCCAA	3412-3429
Modified E2 degenerate forward primer 2	CCGCCGCGACCCATWCCA	3411-3428
Modified E2 degenerate forward primer 3	YCCGCCGCGACCCATWCC	3410-3427
E2 reverse primer	TCGCTGGATAGTCGTCTGTGTT	3475-3454
E2-specific probe	TET-CGTCGCCTTGGGCACCGAA-TAMRA	3433-3451
E2 degenerate probe	TET-CGTCGCCTTGGGCACCRAA-TAMRA	3433-3451

^aSpecific sets of primers and probes were as follows: for E2, E2-specific forward primer, E2 reverse primer, and E2-specific probe, and for E7, E7 forward and reverse primers and E7-specific probe. Degenerate sets of primers and probes were as follows: for E2, E2 degenerate forward primer, E2 reverse primer, and E2 degenerate probe, and for E7, E7 forward and reverse primers and E7 degenerate probe. In modified degenerate sets, the E2 degenerate forward primer was replaced with a modified E2 degenerate forward primer.

^bW, A or T; R, A or G; and Y, T or C. FAM, 6-carboxyfluorescein; MGBNFQ, minor groove binding nonfluorescent quencher; TET, tetrachloro-6-carboxy-fluorescein; TAMRA, 6-carboxytetramethylrhodamine.