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. 2009 Jan 14;47(3):689–696. doi: 10.1128/JCM.01914-08

TABLE 3.

PCR detection of treponemes in BDD lesion biopsies, 2003 to 2007

Sample Biopsy date (day/mo/yr) Description Typea Treponeme isolated (no. of isolates)d Result
Specific PCR for group:
Treponema PCR
1 2 3
1 1/12/03 Cheshire, farm 1, cow 167, left hoof Chronic T167b (2) + + + +
2 1/12/03 Cheshire, farm 1, cow 167, right hoof Acute IF + + − +
3 1/12/03 Cheshire, farm 1, cow 13 IF + + − +
T136b (2) + + + +
4 26/1/04 Shropshire, farm 1, cow 136 T136Ec (1)
T136P2c (3)
5 26/1/04 Shropshire, farm 1, cow 52 T52Ab (2) + + + +
26/1/04 T52Bc (1)
6 26/1/04 Shropshire, farm 1, cow 200 T200 (2)c + + + +
7 26/1/04 Shropshire, farm 1, cow 119 T119Ab (2) + + + +
8 13/2/04 Merseyside, farm 1, cow 320 T320Ab (2) + + + +
9 13/2/04 Merseyside, farm 1, cow 380 T380Ab (2) + + + +
10 13/2/04 Merseyside, farm 1, cow 272 T2721Ab (2) + + + +
11 13/2/04 Merseyside, farm 1, cow 355 T3552Bb (3) + + + +
12 26/4/04 Gloucestershire, farm 1, cow 819 G819CBb (3) + + + +
13 26/4/04 Gloucestershire, farm 1, cow 317 IF + + + +
14 23/4/04 Gloucestershire, farm 2, cow 187 IDD G187b (2) + + − +
15 16/5/04 Gloucestershire, farm 3, cow 1 IF + + + +
16 16/5/04 Gloucestershire, farm 3, cow 169 G169Ab (2) + + + +
17 9/7/04 Merseyside, farm 1, cow 323 T323Cb (2) + + + +
18 9/7/04 Merseyside, farm 1, cow 645 T645C3c (2) + + + +
19 28/4/05 Merseyside, farm 2, cow 116 T116Bc (2) + + + +
20 2/9/05 Cheshire, farm 2, cow 100 T100Ac (2) + + + +
21 2/9/05 Cheshire, farm 2, cow 122 T122Ac (2) + + − +
22 1/12/05 Cheshire, farm 2, cow 5 NIA − + − +
23 15/06/06 Cheshire, farm 2, cow 67, left hoof Chronic NIA + + − +
24 15/06/06 Cheshire, farm 2, cow 67, right hoof Chronic NIA + + + +
25 7/11/06 Cheshire, farm 1, cow 577 NIA + + − +
26 20/11/06 Cheshire, farm 3, cow 87 Chronic NIA + + + +
27 20/11/06 Cheshire, farm 3, cow 574 Chronic NIA + + + +
28 20/11/06 Cheshire, farm 3, cow 265 Acute NIA + + − +
29 11/01/07 Lancashire, farm 1, cow 1 NIA + + + +
a

All lesions were on rear hoofs with typical BDD-type manifestation unless otherwise stated.

b

Isolations reported previously.

c

Previously unreported isolations.

d

IF, isolation failed; NIA, no isolation attempted.