TABLE 3.
Comparison of GP5+/6+ PCR and MGP PCR systems for plasmid controls and for all concordant (i.e., positive by both GP5+/6+ and MGP PCRs) clinical samplesa
| HPV type | GP5+/6+ PCR system
|
MGP PCR system
|
No. of concordant positive samples | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean ratio for no. of copies:
|
Mean cutoff | MFI of positive samples (median) | Mean ratio of samples
|
Mean ratio for no. of copies:
|
Mean cutoff | MFI of positive samples (median) | Mean ratio of samples
|
||||||
| 500 | 5 | Positive | Negative | 500 | 5 | Positive | Negative | ||||||
| 16 | 34.9 | 18.6 | 17 | 557 (587) | 32.8 | 0.67 | 67.2 | 43.0 | 14 | 991 (1,017) | 73.4 | 0.69 | 191 |
| 18 | 15.8 | 3.3 | 11 | 130 (129) | 11.8 | 0.50 | 20.5 | 10.3 | 10 | 153 (130) | 16.5 | 0.63 | 62 |
| 31 | 6.1 | 0.5 | 10 | 99 (91) | 9.9 | 0.22 | 26.3 | 3.8 | 5 | 95 (103) | 19.1 | 0.43 | 66 |
| 33 | 4.1 | 0.6 | 10 | 53 (51) | 5.3 | 0.20 | 16.8 | 4.8 | 5 | 57 (38) | 11.4 | 0.41 | 44 |
| 35 | 1.5 | 0.7 | 16 | 19 (19) | 1.1 | 0.52 | 3.5 | 2.3 | 13 | 29 (29) | 2.0 | 0.64 | 2 |
| 35v | 0.6 | 0.3 | 10 | 31 (28) | 3.1 | 0.21 | 2.3 | 1.4 | 5 | 45 (42) | 9.1 | 0.40 | 18 |
| 39 | 3.4 | 0.7 | 20 | 75 (71) | 3.9 | 0.52 | 8.5 | 5.0 | 16 | 57 (52) | 3.6 | 0.66 | 16 |
| 45 | 6.8 | 0.7 | 10 | 95 (86) | 9.5 | 0.46 | 16.6 | 3.3 | 7 | 101 (85) | 13.8 | 0.63 | 34 |
| 51 | 2.9 | 0.44 | 10 | 56 (55) | 5.6 | 0.30 | 26.1 | 13.5 | 10 | 115 (110) | 12.0 | 0.36 | 29 |
| 52 | 4.1 | 0.6 | 11 | 179 (106) | 15.4 | 0.50 | 172.3 | 108.5 | 8 | 1147 (1,118) | 149.0 | 0.66 | 35 |
| 56 | 54.2 | 3.8 | 10 | 374 (440) | 37.4 | 0.21 | 118.8 | 68.4 | 5 | 357 (371) | 71.3 | 0.41 | 46 |
| 58 | 3.9 | 0.5 | 10 | 31 (32) | 3.1 | 0.24 | 7.1 | 1.7 | 5 | 43 (37) | 5.3 | 0.49 | 14 |
| 59 | 43.8 | 19.4 | 10 | 286 (323) | 28.9 | 0.47 | 36.3 | 14.1 | 14 | 350 (147) | 26.8 | 0.36 | 21 |
| 66 | 18.3 | 3.6 | 10 | 352 (274) | 35.0 | 0.57 | 36.3 | 11.4 | 12 | 411 (411) | 29.1 | 0.34 | 40 |
| 68 | 0.5 | 0.4 | 10 | 10 (10) | 1.0 | 0.40 | 5.7 | 3.4 | 12 | 39 (39) | 3.9 | 0.34 | 1 |
| 6b | ND | ND | 35 | 76 (63) | 2.7 | 0.53 | ND | ND | 62 | 101 (84) | 2.0 | 0.48 | 13 |
| 11b | ND | ND | 35 | 123 (141) | 3.5 | 0.50 | ND | ND | 21 | 191 (226) | 9.0 | 0.85 | 6 |
| 42b | ND | ND | 10 | 116 (105) | 11.6 | 0.33 | ND | ND | 6 | 82 (63) | 14.5 | 0.57 | 28 |
| 43b | ND | ND | 10 | 30 (30) | 3.0 | 0.29 | ND | ND | 6 | 46 (46) | 9.2 | 0.53 | 1 |
| 70b | ND | ND | 10 | 96 (83) | 9.6 | 0.29 | ND | ND | 5 | 126 (121) | 23.1 | 0.54 | 16 |
| 73b | ND | ND | 50 | 74 (79) | 1.6 | 0.48 | ND | ND | 44 | 87 (94) | 2.2 | 0.67 | 5 |
| 82b | ND | ND | 246 | 529 (573) | 2.7 | 0.49 | ND | ND | 275 | 532 (514) | 2.2 | 0.61 | 4 |
a
Ratio denotes sample signal/cutoff. ND, not done.
b
These types were not included in the positive controls.