TABLE 1.
PCR primers used in this study
| Genea | Primer sequence (5′ to 3′) | Primer positions | Product size (bp) | Reference |
|---|---|---|---|---|
| 16S rRNA gene | AAAGATGGCATCATCATTCAAC | 184-205 | 279 | 17 |
| TACCGTCATTATCTTCCCCAAA | 441-462 | |||
| cpa | GCTAATGTTACTGCCGTTGACC | 1280-1300 | 324 | 9 |
| TCTGATACATCGTGTAAG | 1585-1602 | |||
| cpb | GCGAATATGCTGAATCATCTA | 737-757 | 196 | 9 |
| GCAGGAACATTAGTATATCTTC | 911-932 | |||
| etx | GCGGTGATATCCATCTATTC | 258-277 | 665 | 9 |
| CCACTTACTTGTCCTACTAAC | 893-913 |
a
cpa, cpb, and etx are the alpha-, beta-, and epsilon-toxin genes of C. perfringens, respectively.