Figure 4.

Reversal of A4tres DBLβC2 recombinant protein binding to ICAM-1 with ICAM-1 antibodies. A4tres DBLβC2-transfected Cos-7 cells were incubated with ICAM-1/Fc coated beads, washed by inversion, and then incubated in the presence or absence of 10 μg/ml domain 1-specific ICAM-1 antibodies (1G12, 15.2, or RR1/1) or normal mouse IgG. Binding was quantified by inspecting 100 positive transfectants and scoring Cos-7 cells with 5 or more beads as positive. The percentage binding has been normalized to binding observed in the presence of normal mouse IgG. Results are the mean of six experiments plus standard error.