Table III:

Diets used in testing of food regimes for Oikopleura dioica culture

Developmental stage
Diet	Day 1	Day 2	Day 3	Day 4	Day 5
A	0.333/0.167	0.333/0.167	0.333/0.167	0.666/0.333	0.666/0.333
B	2/1	2/1	2/1	2/1	2/1
C	4/2	4/2	4/2	4/2	4/2
D	6/3	6/3	6/3	6/3	6/3
E	10/5	10/5	10/5	10/5	10/5
F	2/1	2/1	2/1	4/2	6/3
G	2/1	2/1	2/1	4/2	4/2
H	2/1	2/1	2/1	4/2	4/2
	0/0	0/0	0/1	1/1	1/1
I	2/1	2/1	2/1	4/2	4/2
	0/0	0/0	0/1	1/1	1/1
	ER/ER	ER/ER	ER/ER	ER/ER	ER/ER
J	2/1	2/1	2/1	4/2	4/2
	0/0	0/0	0/1	1/1	1/1
	ER/ER	ER/ER	ER/ER	ER/ER	ER/ER
	Sa/Sb	Sa/Sb	Sa/–	–/–	–/–

Numbers are given in 1000 cells mL⁻¹ as a final concentration in the 6 L culture volume. For diets A to G, only Isochrysis sp. and Chaetoceros calcitrans were used. For example, 2/1 denotes 2000 cells mL⁻¹ of each of the two algal species were added in the morning and 1000 cells mL⁻¹ of each of the two algal species were added in the evening. In the more complex diets H to J, the first row is as above. The second row refers to morning and evening feeding of an additional, larger alga, Rhinomonas reticulata. ER/ER indicates morning and evening feeding of a soluble extract of R. reticulata obtained from the equivalent of a final concentration of 1000 cell mL⁻¹ of this alga. Sa/Sb denotes morning and evening feeding of Synechococcus sp.: “a” indicates a dose of 80 000 cells mL⁻¹ final concentration and “b” indicates 40 000 cells mL⁻¹ final concentration.