Figure 1.—

PCR amplification products from eggs, purified sperm, and somatic tissues of M. trossulus. (A) DNA extracted from eggs. No PCR product was obtained from eggs of one (lane 4) of five females. The product from eggs from a M. galloprovincialis female is shown for comparison (lane F-ga). (B) DNA extracted from sperm after Percoll purification. No clear PCR product was obtained from sperm of one (lane 7) of 10 males. (C) The products from the eggs and the somatic tissues of female no. 1 (lane 1) and from the sperm and the somatic tissues of male no. 8 (lane 8). M: marker DNA. PCR amplification details as in materials and methods.