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. 2009 Mar;181(3):945–963. doi: 10.1534/genetics.108.097675

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Copyright © 2009 by the Genetics Society of America

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Figure 1.— — Degradation of IAA1-LUC in cul1-7. (A) Single-seedling degradation assay. Experiment performed on 7-day-old seedlings. Zero represents the initial luciferase activity of seedlings in the initial plate reading. Values represent averages ± 1 SD from a total of at least 56 seedlings from two independent experiments. T_1/2 (IAA1-LUC) = 15 and 53 min, respectively, for CUL1 and cul1-7. T_1/2 (LUC) = 70 min in CUL1. (B) Pooled-seedling degradation assay. Values represent averages ± SD from a total of 9 replicates, from three independent experiments. T_1/2 (IAA1-LUC) = 21 and 83 min, respectively, for CUL1 and cul1-7. Loss of LUC in CUL1 is not detected.