Table 2.
Evaluation of F VIII RAg stained sections: microvessel density (MVD) and staining intensity (Int)
| Squamous cell carcinoma |
MDA-MB-231 |
MDA-MB-435 |
Colon cancer |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I | II | III | IV | V | I | II | III | IV | V | I | II | III | I | II | III | Mean | S.D. | ||
| Trypsin | MVD | 5 | 20 | 14 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 30 | 21 | 6 | 6.81 | 2.37 |
| Int | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 2 | |||
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| Pepsin | MVD | 22 | 20 | 19 | 19 | 31 | 1 | 4 | 3 | 3 | 3 | 4 | 13 | 7 | 41 | 15 | 15 | 13.75 | 2.85 |
| Int | 2 | 2 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 3 | 3 | |||
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| Glycine | MVD | 17 | 18 | 11 | 10 | 24 | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 14 | 6 | 4 | 6.88 | 1.95 |
| Int | 2 | 2 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 2 | |||
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| Citric | MVD | 8 | 12 | 6 | 9 | 16 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 11 | 0 | 1 | 4.19 | 1.33 |
| Int | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | |||
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| Borate | MVD | 6 | 12 | 7 | 10 | 14 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 14 | 7 | 9 | 5.31 | 1.31 |
| Int | 1 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 2 | |||
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| 0.01 M Tris/EDTA | MVD | 18 | 12 | 15 | 11 | 28 | 1 | 1 | 0 | 0 | 0 | 7 | 6 | 3 | 17 | 4 | 3 | 7.88 | 2.06 |
| Int | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 2 | |||
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| AR10 | MVD | 10 | 8 | 7 | 9 | 12 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 12 | 2 | 13 | 4.88 | 1.26 |
| Int | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 3 | |||
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| 0.01 M Tris/Tween 20 | MVD | 4 | 8 | 5 | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 2.31 | 0.82 |
| Int | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | |||
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| 0.5 M Tris | MVD | 19 | 14 | 11 | 14 | 20 | 1 | 0 | 0 | 0 | 2 | 13 | 3 | 2 | 18 | 5 | 6 | 8.00 | 1.85 |
| Int | 1 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | |||
I–V, site of designated areas for MVD measurement; Int 0, no staining; 1, weak staining; 2, medium staining; 3, strong staining.
The F VIII RAg MVD obtained with pepsin was significantly greater than that obtained using all the other AR methods (p < 0.01). No intratumor microvessel was observed on the section without treatment (not listed in the table). No microvessel was observed in the SKOV3.ip1 ovarian cancer cell line (data not shown).