. Author manuscript; available in PMC: 2009 Mar 4.

Published in final edited form as: Biotech Histochem. 2008 Jun;83(0):179–189. doi: 10.1080/10520290802451085

Table 2.

Evaluation of F VIII RAg stained sections: microvessel density (MVD) and staining intensity (Int)

		Squamous cell carcinoma					MDA-MB-231					MDA-MB-435			Colon cancer
		I	II	III	IV	V	I	II	III	IV	V	I	II	III	I	II	III	Mean	S.D.

Trypsin	MVD	5	20	14	9	3	0	0	0	0	0	0	1	0	30	21	6	6.81	2.37
Trypsin	Int	1	2	1	1	1	0	0	0	0	0	0	1	0	3	2	2

Pepsin	MVD	22	20	19	19	31	1	4	3	3	3	4	13	7	41	15	15	13.75	2.85
Pepsin	Int	2	2	2	1	2	1	1	1	1	1	1	1	1	3	3	3

Glycine	MVD	17	18	11	10	24	0	2	1	0	1	1	1	0	14	6	4	6.88	1.95
Glycine	Int	2	2	1	1	2	0	1	1	0	1	1	1	0	2	2	2

Citric	MVD	8	12	6	9	16	1	0	0	0	1	0	1	1	11	0	1	4.19	1.33
Citric	Int	1	1	1	1	2	1	0	0	0	1	0	1	1	2	0	1

Borate	MVD	6	12	7	10	14	0	1	0	0	0	1	3	1	14	7	9	5.31	1.31
Borate	Int	1	1	1	2	1	0	1	0	0	0	1	1	1	3	2	2

0.01 M Tris/EDTA	MVD	18	12	15	11	28	1	1	0	0	0	7	6	3	17	4	3	7.88	2.06
0.01 M Tris/EDTA	Int	2	1	1	1	2	1	1	0	0	0	1	1	1	3	2	2

AR10	MVD	10	8	7	9	12	0	1	0	0	0	2	2	0	12	2	13	4.88	1.26
AR10	Int	1	1	1	1	1	0	1	0	0	0	1	1	0	2	2	3

0.01 M Tris/Tween 20	MVD	4	8	5	7	9	0	0	0	0	0	1	0	0	3	0	0	2.31	0.82
0.01 M Tris/Tween 20	Int	1	1	1	1	1	0	0	0	0	0	1	0	0	1	0	0

0.5 M Tris	MVD	19	14	11	14	20	1	0	0	0	2	13	3	2	18	5	6	8.00	1.85
0.5 M Tris	Int	1	2	2	1	1	1	0	0	0	1	1	1	1	2	2	2

I–V, site of designated areas for MVD measurement; Int 0, no staining; 1, weak staining; 2, medium staining; 3, strong staining.

The F VIII RAg MVD obtained with pepsin was significantly greater than that obtained using all the other AR methods (p < 0.01). No intratumor microvessel was observed on the section without treatment (not listed in the table). No microvessel was observed in the SKOV3.ip1 ovarian cancer cell line (data not shown).