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. Author manuscript; available in PMC: 2009 Mar 4.
Published in final edited form as: Biotech Histochem. 2008 Jun;83(0):179–189. doi: 10.1080/10520290802451085

Table 2.

Evaluation of F VIII RAg stained sections: microvessel density (MVD) and staining intensity (Int)

Squamous cell carcinoma
MDA-MB-231
MDA-MB-435
Colon cancer
I II III IV V I II III IV V I II III I II III Mean S.D.

Trypsin MVD 5 20 14 9 3 0 0 0 0 0 0 1 0 30 21 6 6.81 2.37
Int 1 2 1 1 1 0 0 0 0 0 0 1 0 3 2 2

Pepsin MVD 22 20 19 19 31 1 4 3 3 3 4 13 7 41 15 15 13.75 2.85
Int 2 2 2 1 2 1 1 1 1 1 1 1 1 3 3 3

Glycine MVD 17 18 11 10 24 0 2 1 0 1 1 1 0 14 6 4 6.88 1.95
Int 2 2 1 1 2 0 1 1 0 1 1 1 0 2 2 2

Citric MVD 8 12 6 9 16 1 0 0 0 1 0 1 1 11 0 1 4.19 1.33
Int 1 1 1 1 2 1 0 0 0 1 0 1 1 2 0 1

Borate MVD 6 12 7 10 14 0 1 0 0 0 1 3 1 14 7 9 5.31 1.31
Int 1 1 1 2 1 0 1 0 0 0 1 1 1 3 2 2

0.01 M Tris/EDTA MVD 18 12 15 11 28 1 1 0 0 0 7 6 3 17 4 3 7.88 2.06
Int 2 1 1 1 2 1 1 0 0 0 1 1 1 3 2 2

AR10 MVD 10 8 7 9 12 0 1 0 0 0 2 2 0 12 2 13 4.88 1.26
Int 1 1 1 1 1 0 1 0 0 0 1 1 0 2 2 3

0.01 M Tris/Tween 20 MVD 4 8 5 7 9 0 0 0 0 0 1 0 0 3 0 0 2.31 0.82
Int 1 1 1 1 1 0 0 0 0 0 1 0 0 1 0 0

0.5 M Tris MVD 19 14 11 14 20 1 0 0 0 2 13 3 2 18 5 6 8.00 1.85
Int 1 2 2 1 1 1 0 0 0 1 1 1 1 2 2 2

I–V, site of designated areas for MVD measurement; Int 0, no staining; 1, weak staining; 2, medium staining; 3, strong staining.

The F VIII RAg MVD obtained with pepsin was significantly greater than that obtained using all the other AR methods (p < 0.01). No intratumor microvessel was observed on the section without treatment (not listed in the table). No microvessel was observed in the SKOV3.ip1 ovarian cancer cell line (data not shown).