Fig. 2.

Cyclosporin A (CsA) protects mice from pyrazole (PY) plus lipopolysaccharide (LPS)-induced oxidative stress in liver. (A) Immunohistochemical staining of 4-hydroxynonenal (HNE) protein adducts and 3-nitrotyrosine (3-NT) protein adducts in liver tissue were carried out as described in Materials and Methods. Circles showed positive staining for HNE or 3-NT in liver sections. The images are representative of results from 6 control-, 9 PY+LPS-, and 9 PY+LPS+CsA-treated mice. (B) GSH levels in liver homogenate were assayed with the fluorometric substrate o-phthalaldehyde. *P<0.05, vs. control mice, #P<0.005 and P<0.001, vs. control and PY-treated mice, respectively, $ P<0.05, P<0.001, P>0.05, vs. control, PY-, PY plus LPS-treated mice, respectively. (C) Liver SAM levels were assayed by high performance liquid chromatography. No significant difference was found between the 4 groups.