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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2008 Oct 31;46(3):406–413. doi: 10.1016/j.freeradbiomed.2008.10.037

Fig. 5.

Fig. 5

Western blot analysis of mitochondrial BCL-2 family protein levels. One hundred μg of liver mitochondrial protein was subjected to 18% sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotted with mouse anti-BCL-2 (C-2), rabbit anti-BAX (Ab-1), anti-BID (FL-195), anti-BCL-xS/L (L-19) antibodies and anti-SOD2 (G20) antibody as a mitochondrial loading control. Bands were quantified by the ImageJ image processing program. (A) BCL-2, *P<0.05, vs. control. (B) BAX, *P<0.005, vs. control. (C) BID, *P<0.005, #P<0.001, vs. control. (D) BCL-Xs/L, *P<0.005, vs. control. Numbers under the blot refer to the mean ± SD of the ratio of BCL-2, BAX, BID, BCL-xL and BCL-xS to SOD2 and are from 3 mice in each group.

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