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. 2009 Mar 4;106(12):4923–4928. doi: 10.1073/pnas.0809613106

Fig. 2.

Fig. 2.

Loss of Cyp1a1 and Cyp1a2 induction in dioxin-treated DREC−/− mice. Total RNAs were isolated from the livers of WT, DREC+/−, and DREC−/− mice. The mice were given a single injection of DMSO vehicle alone or 64 μg/kg of dioxin in DMSO and were killed 5 days later. (A) Relative fold induction of Cyp1a1 and Cyp1a2 mRNA by dioxin. The mRNA levels were quantified by the Molecular Dynamics Storm system, and the measured Cyp1a1 and Cyp1a2 mRNA levels were normalized to the GAPDH mRNA level. Results are expressed as relative mRNA level compared with DMSO-treated WT mice. (B) EROD activity (indicating Cyp1a1 enzyme activity) and MROD activity (indicating Cyp1a2 activity), expressed as relative fluorescence units per mg of microsome protein. Each group contained more than 4 mice. Open bars, DMSO treatment; closed bars, dioxin treatment. Error bars represent SE. a, Significantly different relative to the DMSO-treated WT mice (P < .05). b, Significantly different relative to the dioxin-treated WT mice (P < .05).