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. 2009 Feb 11;106(9):3384–3389. doi: 10.1073/pnas.0808300106

Fig. 1.

Fig. 1.

Posttranscriptional repression of let-7 expression by Myc correlates with Lin-28B induction. (A and B) qPCR analysis of the abundance of pri-miRNAs (A) and mature let-7g (B) in P493-6 cells with high or low Myc expression. (C) RT-PCR analysis of Lin-28 and Lin-28B expression in P493-6 cells. DNA Plasmids bearing the ORF of Lin-28 or Lin-28B were used as templates for positive controls. (D) qPCR analysis of Lin-28B expression in P493-6 cells. (E) Western blot of Lin-28B abundance in P493-6 cells after removal of tet. Signals were normalized to α-tubulin levels before calculating fold changes. (F) qPCR analysis of Lin-28B expression in murine B cell lymphomas. Two independent tumors from MycON and MycOFF neoplasms were assayed. (G) qPCR analysis of Lin-28B expression in Ras-transformed p53-null colon carcinomas. Four independent tumors from Ras/Myc and Ras-only neoplasms were assayed. For all qPCR analyses, error bars represent standard deviations derived from 3 independent measurements.