Fig. 3.

Lin-28B knockdown reverses let-7 repression and slows proliferation. (A and B) P493-6 cells were grown the absence of tet (high Myc state) and transfected with 2 different siRNAs targeting Lin-28B or negative control siRNA. qPCR was used to measure Lin-28B (A) or mature let-7g abundance (B). (C) Proliferation of P493-6 cells after siRNA delivery. Error bars represent standard deviations of measurements of triplicate transfections. (D) Proliferation of P493-6 cells after transfection of siRNA and miRNA inhibitors. Error bars represent standard deviations of measurements of triplicate transfections. P value calculated by 2-tailed t test.