Fig. 2.

In vivo synthesis of sterols (A) and fatty acids (B) in 24-hydroxylase KO and AD mice. Mice of the indicated genotypes (11- to 14-month-old females, 10 per group) were maintained on a normal chow diet containing 0.02% cholesterol. On the day of the experiment, each animal was injected peritoneally with 50 mCi of tritium-labeled water; 1 h later tissues were removed and processed for measurement of radio-labeled sterols and fatty acids as described in the SI. Student's t test was performed to assess differences between experimental groups. In (A), KO values were significantly (P = 0.022) lower than WT values; KO/AD values were not significantly (P = 0.06) lower than WT/AD values. Error bars indicate SEM values.