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Insoluble Aβ peptide levels in 24-hydroxylase KO and AD mice. Cortical (A and B) and hippocampal (C and D) Aβ peptide levels in mice (≥5 animals per group) of the indicated genotype, sex, and age were quantified by ELISA as described in Materials and Methods and the SI. Tissues were homogenized in RIPA buffer containing protease inhibitors. Insoluble Aβ peptides were isolated by centrifugation and solubilized with 70% formic acid before measurement. Error bars indicate SEM values.
