Fig. 4.
Stimulated 5-HT release and intracellular Ca2+ in RIN14B cells. Ionomycin (10 μM) induced the release of 5-HT from RIN14B cells (A). The dose-response curves of TRPA1 agonist-induced increase in [Ca2+]i in RIN14B cells (B). Blockade of the AITC (30 μM)-evoked increase in [Ca2+]i induced by ruthenium red (3 μM) in RIN14B cells (C). TRPA1 agonist-induced release of 5-HT from RIN14B cells (D). Effects of the TRPA1 inhibitor ruthenium red (30 μM) on the AITC (300 μM)- or CA (300 μM)-induced 5-HT release from RIN14B cells (E). Role of Ca2+ in the TRPA1 agonist-induced release of 5-HT from RIN14B cells (F). The cells were stimulated by AITC (300 μM) or CA (300 μM) in HBSS (open column) and Ca2+-free HBSS (solid column). All data represent the mean ± S.D. (n = 3). **, P < 0.01; ***, P < 0.001 vs. control group (Dunnett's test for D and Student's t test for A, C, E, and F).