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. 2009 Feb 9;106(9):3077–3082. doi: 10.1073/pnas.0813247106

Fig. 3.

Fig. 3.

Rad52 permits formation of complement-stabilized D-loops in the presence of RPA, but E. coli RecO does not. (A) Gel comparing the ability of Rad52 and RecO to mediate cs-D-loop formation in the presence of RPA (0.4 μM). Standard reactions were performed by using Rad52 or RecO: lanes 1–5: 0, 0.4, 0.8, 1.2, and 1.6 μM, Rad52, respectively; lanes 6–10: 0, 0.4, 0.8, 1.2, and 1.6 μM RecO, respectively. The NaCl added by Rad52 and RecO storage buffers was 80 mM. The positions of cs D-loops and free oligonucleotides are indicated. (B) Quantification of cs-D-loop formation. The percentage of cs-D-loops from experiments as shown in A was expressed with respect to the limiting scDNA concentration.