Skip to main content
. 2009 Feb 9;106(9):3077–3082. doi: 10.1073/pnas.0813247106

Fig. 4.

Fig. 4.

Rad52 cannot efficiently form complement-stabilized D-loop in the presence of E. coli SSB. (A) Gel comparing the ability of Rad52 to mediate cs-D-loop formation in the presence of RPA or SSB. Standard reactions were performed by using RPA or SSB: lanes 1–4, RPA (0.4 μM) and 0, 0.4, 0.8, and 1.2 μM Rad52, respectively; lanes 5–8, SSB (0.4 μM) and 0, 0.4, 0.8, and 1.2 μM Rad52, respectively. The NaCl added by the Rad52 storage buffer was 80 mM. The positions of cs-D-loops and free oligonucleotides are indicated. (B) Quantification of cs-D-loop formation. The percentage of cs-D-loops from experiments as shown in A was expressed with respect to the limiting scDNA concentration.