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. 2009 Feb 9;106(9):3077–3082. doi: 10.1073/pnas.0813247106

Fig. 5.

Fig. 5.

Rad52-mediated second-end capture as part of DSBR. (Step I) RPA binds to the 3′-terminated ssDNA tails of the resected DSB. (Step II) Rad52 binds to the RPA–ssDNA complex. (Step III) Rad52 mediated displacement of RPA by Rad51 to form a Rad51 nucleoprotein filament (Left end); Rad54 binds and stabilizes the Rad51 nucleoprotein filament. (Step IV) The Rad54–Rad51–DNA nucleoprotein filament catalyzes DNA strand invasion to form a D-loop, displacing the resident strand in the target dsDNA; the displaced strand is bound by RPA and Rad52. (Step V) Rad54 clears Rad51; the invading 3′ end primes DNA synthesis; extension causes the displaced strand to advance until an ssDNA region complementary to second resected DNA end is exposed; Rad52 mediates annealing of the RPA-complexed displaced strand to second end (Right end). (Step VI) Additional DNA synthesis, branch migration, and ligation generate dHJs. (Step VII) Resolution to produce cross-overs or non-cross-overs.