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Functional screening for RNA cap-forming functions encoded by SARS-CoV. (A) YBS40 could be complemented by SARS-CoV-nsp14 and the positive control pYX232-HCM1 but not by other nonstructural proteins of SARS-CoV. (B) The nsp14 of both SARS-CoV and TGEV could rescue the growth of YBS40 on 5-FOA medium. The known N7-MTases including nsP1 of SFV and NS5-MTase domain of WNV could not substitute for the yeast N7-MTase activity.
