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. 2009 Feb 13;106(9):3461–3466. doi: 10.1073/pnas.0808933106

Fig. 3.

Fig. 3.

Biochemical and phenotypic analysis of EAL-domain point mutants. (A) Membrane preparations were assayed for in vivo levels of LapD by Western blot. (B) c-di-GMP binding by LapD variants and WT LapD. (C) Biofilm assay performed on ΔlapD complemented with pLapD or EAL-domain mutants. (D) Strains with the ΔlapD, WT, or K446A allele of lapD are compared for biofilm formation when the DGC PA1107 is expressed.