Fig. 5.

Expression and function of retroviral TCR in T-hybridoma cells. Supernatant from the retrovirus-producing GP+E86 cells were collected for infection of the TCR-deficient BW-1 and 4G4.CD4 T-hybridoma cells. (A) BW-1 cells were stained with TCRβ antibody. Flow cytometry shows the expression of all 4 TCR pairs (αβ1, αβ7, αβ11, and αβ16) on infected (GFP-positive) cells. No TCR expression was detected on BW-1 cells infected by vector only. (B and C) The TCR-bearing and vector-only infected T-hybridoma cells were sorted for GFP⁺TCRβ⁺ (groups αβ1, -7, -11, and -16) or GFP⁺ (vector) populations and stimulated with different antigens presented by APC. Supernatant from stimulated BW-1 (B) or 4G4.CD4 (C) cells were harvested at 48 h, and IL-2 production was measured by ELISA.