Fig. 2.

Induction of miR-145 by p53. (A) MCF-7 cells were transduced with adenoviral pAd-GFP (Vector) or pAd-GFP-p53 (p53) for 24 h and then harvested for extraction of protein or RNA. Insert, detection of p53 expression. (B) HCT-116 (p53−/−) or HCT-116 (+/+) were treated with Doxo at 0.5 or 1.0 μg/ml for 16 h. The cells were separately harvested for protein or RNA. p21 was used as a positive control. (C) MCF-7 cells were transiently transfected with vector (pCMV) or mutant p53 (R175H) and incubated for 24 h. Insert, detection of mutant p53. Values in A, B, and C are means ± SE of 3 separate experiments and normalized to their respective controls as 1. **, P < 0.01.