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α-GalCer promotes the generation of long-term CD8+ T cell memory. The B6 mice were immunized with iIAV ± α-GalCer and killed 6 weeks later. Splenocytes were harvested and analyzed for the percentage (A) and absolute number (B) of tetramer-positive CD8+ CTLs. (C) The proportion of TCM vs. TEM cells was evaluated at early and late time points after immunization. Results are expressed as mean ± SD for groups of 5 mice. *P ≤ 0.05; **P < 0.01
