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. Author manuscript; available in PMC: 2009 Mar 4.
Published in final edited form as: Dev Dyn. 2008 Oct;237(10):2830–2843. doi: 10.1002/dvdy.21702

Fig. 5.

Fig. 5

Protein extracts from S. macrurus blastema (SmBE) enhances the proliferation of C2C12 myoblasts. Quantification of bromodeoxyuridine (BrdU) -labeled cells. After C2C12 myoblasts were induced to differentiate in differentiation medium (DM, they were re-plated at low density and treated with either DM only (control), adult muscle extract (AM), or SmBE (1-week and 2-week) at 0.05 mg/ml for 3 days. Cells were incubated with BrdU for the last 12 hr of the 3-day treatment in each condition. Cells from 10 different microscopic fields with ≥ 100 cells per field were counted and the percentage of BrdU-labeled cells was calculated in each field. Data are the means ± SD. Student t-test was used to compare the effect of extract with control. Asterisk (*) indicates a difference at P < 0.05.