Figure 3. Expression of FPRL1 in neutrophils.
A): FPRL1 gene expression in adult and neonatal neutrophils was analyzed by reverse transcription PCR. PCR products were visualized on an agarose gel stained with ethidium bromide and quantified by densitometry. Data were normalized to β-actin expression. Each bar represents mean ± SE (n=3). Neutrophils from adults (black lines) and neonates (red lines) were incubated with IgG control (B) or anti-FPRL1 antibody (C), followed by FITC-labeled secondary antibody, and analyzed by flow cytometry. One representative histogram from three experiments is shown.