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. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: Pediatr Res. 2008 Oct;64(4):393–398. doi: 10.1203/PDR.0b013e318180e4af

Neutrophils from adults (■) and neonates were analyzed immediately after isolation (0 hr) or were incubated with PGE2 (300 nM) or medium control for 5 hr, followed by incubation with LXA4 (300 nM) or medium control for an additional 4 hr. Real time PCR was performed using specific primers for PPAR-γ (A) or NGAL (B), and data normalized to glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) expression. Data are expressed as mean ± SE (n = 6−8). *Significantly different (p<0.05) from adult; **Significantly different (p<0.05) from 0 hr; Significantly different (p<0.05) from untreated 9 hr (PGE2−/LXA4−); ^‡Significantly different (p<0.05) from PGE2 alone (PGE2+/LXA4−); Significantly different (p<0.05) from LXA4 alone (PGE2−/LXA4+).

Inline graphic — Neutrophils from adults (■) and neonates were analyzed immediately after isolation (0 hr) or were incubated with PGE2 (300 nM) or medium control for 5 hr, followed by incubation with LXA4 (300 nM) or medium control for an additional 4 hr. Real time PCR was performed using specific primers for PPAR-γ (A) or NGAL (B), and data normalized to glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) expression. Data are expressed as mean ± SE (n = 6−8). *Significantly different (p<0.05) from adult; **Significantly different (p<0.05) from 0 hr; Significantly different (p<0.05) from untreated 9 hr (PGE2−/LXA4−); ^‡Significantly different (p<0.05) from PGE2 alone (PGE2+/LXA4−); Significantly different (p<0.05) from LXA4 alone (PGE2−/LXA4+).