Table 2.
Quantification of Verticillium dahliae biomass in Arabidopsis gene-silencing mutants by real-time PCR comparison of V. dahliae internal transcribed spacer (ITS) transcript levels (as a measure for fungal biomass) relative to Arabidopsis RuBisCo transcript levels (for equilibration) at 19–29 d post-inoculation with V. dahliae strain JR2
| Gene name | Genotype | Symptom displaya | Biomass fold changeb | Significancec |
| Col-0 | – | 1 | – | |
| AGO1 | ago1-27 | Reduced | 0.007 | P <0.1 |
| AGO7 | ago7-2 | Enhanced | 3.174 | P <0.2 |
| DCL2 | dcl2-1 | Similar | 0.829 | No |
| DCL4 | dcl4-2 | Enhanced | 2.422 | P <0.05 |
| HEN1 | hen1-6 | Reduced | 0.045 | P <0.1 |
| HST | hst1-1 | Reduced | 0.039 | P <0.05 |
| NRPD1a/SDE4 | nrpd1a-3 | Enhanced | 1.816 | P <0.2 |
| RDR2 | rdr2-4 | Enhanced | 2.701 | P <0.05 |
| RDR6/SDE1/SGS2 | sgs2-1 | Enhanced | 2.279 | P <0.05 |
| rdr6-15 | Enhanced | 3.286 | P <0.05 | |
| SDE3 | sde3-4 | Similar | 1.674 | No |
| SGS1 | sgs1-1 | Enhanced | 3.729 | P <0.05 |
| SGS3/SDE2 | sgs3-1 | Enhanced | 2.938 | P <0.05 |
Symptom display upon V. dahliae inoculation when compared with Col-0 (also see Fig. 3).
The relative average fungal biomass is indicated as relative fold-change when compared with fungal biomass in V. dahliae-inoculated Col-0 plants of which the average fungal biomass was set to one.
Statistically significant differences are given as P-values according to a Student's t test with a 95% to an 80% confidence interval (P <0.05–0.2).