Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jan 6;60(2):663–678. doi: 10.1093/jxb/ern322

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

PMC Copyright notice

Fig. 1. — Expression of AA biosynthetic genes during tomato fruit ripening. (A) RNA blot analysis of AA biosynthetic genes. (B) The main and alternative AA biosynthetic routes. (C) Ascorbate levels during development and ripening. Total RNA (20 μg) isolated from different stages ranging from anthesis, the first, second, and third weeks from anthesis (FL) (W1, W2, and W3), IG (immature green), MG (mature green), BR (breaker), TR (turning), PK (pink), LR (light red), R (red), to RR (ripe red) was fractionated in formaldehyde denaturing agarose gels, transferred to nylon membranes, stained with 0.04% methylene blue (to observe the equality of loading), and hybridized with radiolabelled probes for AA biosynthetic genes. All experiments were performed in triplicate.