Fig. 4.
Expression of AA oxidation and recycling enzymes in response to ethylene and various stresses. Tomatoes were grown in the field and harvested at the mature green stage. After subjecting fruit to various stresses, total RNA was isolated, fractionated in denaturing agarose gels, transferred to Hybond N-membranes, and hybridized with specific 32P-radiolabelled probes. Staining with 0.04% methylene blue was performed to verify the uniformity of RNA loading on the gel. All experiments were performed in triplicate.