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. 2009 Jan 6;60(2):615–627. doi: 10.1093/jxb/ern311

Fig. 9.

Fig. 9.

Influence of 2-ME on prolamin–GFP fusion protein solubilization. In the experiment shown in A, proteins from seeds of WT (lanes 1 and 4), 35S:GFP (lanes 2 and 5), and 35S:Pro-GFP (lanes 3 and 6) plants were extracted with 5% (v/v) 2-ME (lanes 1–3) or without 2-ME (lanes 4–6). In B, homogenates from leaves of WT (lanes 1 and 4), 35S:GFP (lanes 2 and 5), and 35S:Pro-GFP (lanes 3 and 6) plants were subjected to centrifugation to obtain the 15 000 g pellet (P15) and the 15 000 g supernatant (S15). Proteins in each fraction were extracted with 5% (v/v) 2-ME (lanes 1–3) or without 2-ME (lanes 4–6). The proteins were separated by SDS–PAGE and immunoblotted with anti-GFP antibodies. The upper bands (open arrowheads) and lower bands (filled arrowheads) correspond to prolamin–GFP and GFP, respectively.