Fig. 4.

Immunogold EM labelling of α-TIP and 8S globulin in developing mung bean. Ultrathin sections prepared from high-pressure frozen/freeze-substituted developing mung bean seeds were immunogold-labelled with either α-TIP antibodies (A) or 8S globulin antibodies (B), as indicated. Arrows and arrowheads indicate examples of gold particles on the tonopalst and lumen of protein storage vacuole (PSV) respectively. α-TIP, alpha-tonoplast intrinsic protein; PSV, protein storage vacuole. Scale bar=200 nm.