Fig. 5.

Immunogold EM double-labelling study. Ultrathin sections prepared from high-pressure frozen/freeze-substituted developing mung bean seeds were immunogold double-labelled with pairs of antibodies of either SBP1a (15 nm) and α-TIP (10 nm) (A) or SBP1a (15 nm) and 8S globulin (B). Arrows and arrowheads indicate examples of gold particles on the tonoplast and lumen of the protein storage vacuole (PSV), respectively. Scale bar=200 nm.