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. 2008 Oct 24;68(4):599–602. doi: 10.1136/ard.2008.097378

Figure 1.

Figure 1

A. Evaluation by 3-(4,5-dDimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test of cell proliferation in human normal fibroblasts (Fb) untreated (control) and treated for 24 h with endothelin-1 (ET-1) (10−7 M), 17β-oestradiol (E2) (10−10 M), E2 (10−10 M) with ET-1 (10−7 M), testosterone (T) (10−9 M) or T (10−9 M) with ET-1 (10−7 M). **p<0.01; ***p<0.001. B. Evaluation by western blot analysis of proliferating cell nuclear antigen (PCNA) expression in human normal Fb untreated (control) and treated for 24 h with ET-1 (10−7 M), E2 (10−10 M), E2 (10−10 M) with ET-1 (10−7 M), T (10−9 M) or T (10−9 M) with ET-1 (10−7 M). C. Evaluation by MTT test of cell proliferation in human systemic sclerosis fibroblasts (SSc Fb) untreated (control) and treated for 24 h with ET-1 (10−7 M), E2 (10−10 M), E2 (10−10 M) with ET-1 (10−7 M), T (10−9 M) or T (10−9 M) with ET-1 (10−7 M).