Figure 5.

Effect of temperature on the fate of α1-ATZ in CJZ12B cells. Cells were incubated for 12 h at normal growth temperature (37°C) or adjusted temperatures (27 or 42°C) and subjected to pulse-chase radiolabelling exactly as described in Materials and Methods. The relative migration of the 55-kDa α1-ATZ polypeptide in the EC is indicated by the arrowhead at the right margin. A 65-kDa polypeptide which coprecipitates with α1-ATZ in cell lysates at 42°C is indicated by an asterisk in the left margin.