Skip to main content
. Author manuscript; available in PMC: 2010 Jan 7.
Published in final edited form as: Vaccine. 2008 Nov 7;27(2):213–222. doi: 10.1016/j.vaccine.2008.10.046

Fig. 3.

Fig. 3

rWNV-E adsorption to to Alhydrogel™ and stability study. (A) SDS-PAGE analyses of rWNV-E antigen before (lane 1, lower arrow) and after (lane 2, higher arrow) it was adsorbed to Alhydrogel™ for one hour at room temperature. The gel was stained with Coomassie blue. (B) Competitive ELISA analyses compared rWNV-E freshly adsorbed to Alhydrogel™ (rWNV-E control) with rWNV-E adsorbed to Alhydrogel™ and stored at 4°C or at 37° for three weeks, three months or eight months. EC50 values for control rWNV-E control and formulated rWNV-E stored at 37 °C are indicated by dashed vertical lines, and differences in value between the two were shown by horizontal arrows ( EC50).