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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Cell. 2007 Oct 19;131(2):286–299. doi: 10.1016/j.cell.2007.08.041

Figure 2. Recycling of post-TCs and assembly of 48S initiation complexes on recycled mRNA promoted by initiation factors.

Figure 2

(A, C) Ribosomal association of [32P]MVHL-STOP mRNA after incubation of pre-TCs with eRFs and eIFs, as indicated, assayed by sucrose gradient centrifugation. The positions of 40S subunits and 80S ribosomes are indicated. (B) Toe-printing analysis of the 40S-containing fraction (panel A, circles) obtained after incubating pre-TCs with eRFs and eIFs. The positions of full-length cDNA and of toe-prints that correspond to 48S complexes are indicated. Lanes C, T, A, G depict cDNA sequences corresponding to MVHL-STOP mRNA.