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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Cell. 2007 Oct 19;131(2):286–299. doi: 10.1016/j.cell.2007.08.041

Figure 4. Association of eIF3 with recycled 40S subunits and dissociation of P site deacylated tRNA.

Figure 4

(A) Ribosomal association of [32P]eIF3 after incubation of pre-TCs assembled on MVHL-STOP mRNA with different combination of eRFs and eIFs, as indicated, assayed after sucrose gradient centrifugation by Cerenkov counting and gel electrophoresis (right panel). (B, C) Ribosomal association of [32P]tRNALeu after incubation of pre-TCs assembled in the presence of Leu-[32P]tRNALeu with eRFs and eIFs, as indicated, assayed after sucrose gradient centrifugation by Cerenkov counting. The positions of 40S subunits and 80S ribosomes are indicated.