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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Cell. 2007 Oct 19;131(2):286–299. doi: 10.1016/j.cell.2007.08.041

Figure 5. Dissociation of mRNA from post-TCs.

Figure 5

(A–C, F) Ribosomal association of [32P]MVHL-STOP mRNA after incubation of pre-TCs with eRFs, eIFs and puromycin, as indicated, assayed after sucrose gradient centrifugation by Cerenkov counting. The positions of 40S subunits, 80S ribosomes and mRNPs are indicated. (D,E, lower panel of F) Toe-printing analysis of 40S-containing fractions shown on panels A–C (E), and of 80S-containing fractions shown on panels A–C and F (D and F, respectively). The positions of full-length cDNA and of toe-prints that correspond to ribosomal complexes are indicated. Lanes C, T, A, G depict cDNA sequences corresponding to MVHL-STOP mRNA.