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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Biochemistry. 2002 Jan 8;41(1):266–273. doi: 10.1021/bi015718l

FIGURE 3.

FIGURE 3

Determination of equilibrium transfer levels of AV-GalCer attained with bovine brain SPM-POPC mixed SUVs. Triton X-100 was added to the transfer reaction after the transfer reached equilibrium to establish the AV-GalCer fluorescence intensity expected at infinite dilution (31). The fluorescence levels after detergent addition, marked with an X (minus a Triton X-100 blank), correspond to the total fluorescence for the reaction containing 4 nmol (1 mol %) AV-GalCer. These fluorescence values were used to determine the transfer rates for the GLTP-mediated AV-GalCer transfer between donor and acceptor vesicles.