TABLE 1. Rhodopsin exists as a monomer in rHDL particles.

Rhodopsin·rHDL particles were resolved on a size exclusion column and then purified on a ConA-Sepharose column. The load, flow-through, and three serial elutions from the ConA-Sepharose column were assayed for absorbance at λ = 280 nm and λ = 500 nm. ConA-Sepharose column eluates were pooled and re-resolved on a size exclusion column in order to confirm that the size of the rhodopsin·rHDL particles remained the same before and after processing by the ConA-Sepharose column. The average A₂₈₀A₅₀₀ ratios of all fractions across the peak were calculated as described under “Experimental Procedures.”

Fraction	A280	A500	[Rhodopsin]:[apoAI]
ConA-Sepharose column
Load	1	0.15	0.15
Flow-through	0.57	0.02	0.035
Elute 2	0.12	0.04	0.47
Elute 3	0.07	0.02	0.47
Elute 4	0.04	0.01	0.42
SEC column
Load			0.46
SEC average			0.47 ± 0.05