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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Nat Struct Mol Biol. 2008 Jan 27;15(2):170–176. doi: 10.1038/nsmb.1381

Figure 4. Leading-strand synthesis by Pol III holoenzyme with DnaB.

Figure 4

Leading-strand synthesis by Pol III holoenzyme coupled with DnaB helicase. (a) Duplex λ DNA with a premade replication fork is stretched under laminar flow, and Pol III holoenzyme, DnaB and DnaC are introduced into the chamber. Synthesis is observed as a shortening of the tethered DNA. (b) Examples of leading-strand synthesis traces, in the absence (left) and presence (right) of DnaG. (c) Distribution of leading-strand synthesis processivities, fit with a single-exponential decay. Leading-strand synthesis was observed as single shortening events with an average processivity of 10.5 kb. (d) Distribution of synthesis rates, fit with a Gaussian distribution.

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