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. 2009 Mar 10;4(3):e4773. doi: 10.1371/journal.pone.0004773

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Koutsonanos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Splenocytes were stimulated with inactivated whole virus for 6 days in the presence of ConA medium to assess the generation of anti-influenza IgG memory B cell by ELISPOT, (average±s.e). ConA (d1): ASC cells stimulated with ConA medium and enumerated on day 1.Virus+ConA (d1): memory B cells stimulated with influenza virus as an antigen and ConA medium and enumerated on day 1. Virus+ConA (d6): memory B cells stimulated with influenza virus and ConA medium and enumerated on day 6.